Direct detection of pcr product is monitored by measuring the increase in. Sybr green pcr master mix and sybr green rt pcr reagents kit user guide 9 1 product information purpose of the kit the sybr green pcr master mix is a convenient premix of the components except primers template and water necessary to perform real time pcr using sybr green i dye. Master mix the sybr green pcr master mix is supplied in a 2x concentration and contains sufficient reagents to perform 200 50 µl reactions.
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